PCR can be a bit tricky, sometimes leading to unrepeated and unreliable results. In this article, discover how to remain in control of PCR protocols.

The researchers compared the government-supplied Innova LFD with a known RT-PCR protocol with a wide range of samples known to be positive for SARS-CoV-2. In total, they used 62 stored samples ...

Emulsion PCR made easy Method Summary Here we describe a simplified method for direct breakage of the emulsion using the DNA binding buffer (containing guanidine hydrochloride and isopropanol) that is ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

This book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the setting-up of a PCR ...

PCR-based methods offer advantages over more traditional diagnostic tests, in that organisms do not need to be cultured prior to their detection and protocols are highly sensitive and rapid.